Guinea Pig Leukocyte Cultures
نویسندگان
چکیده
High titers of interferon were induced by the psittacosis agent in guinea pig leukocyte cultures. Optimal yields of interferon were produced from freshly prepared guinea pig leukocyte cultures containing 4 x 106 cells per ml inoculated with a psittacosis/cell multiplicity of 1.0 and incubated at 35 C for 24 hr. Leukocytes obtained from 2to 4-month-old guinea pigs produced 20 times more interferon than leukocytes from 3to 4-week-old animals. The biological activity of interferon and the kinetics of its production from psittacosis-infected guinea pig leukocyte cultures
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